Our Quick-Glia™ iPSC glial differentiation kits allow researchers to quickly, easily, and efficiently differentiate their desired iPS cell line into a variety of glial cell types, including astrocytes.
Quick-Glia™ Differentiation Kits & Media
Although human iPSCs have many advantages over existing models, a drawback is that differentiation has historically been a very time and labor intensive process. Our Quick-Glia™ differentiation kits overcome this obstacle by utilizing our proprietary transcription factor-based technology that allows for rapid and reproducible differentiation of stem cells into a variety of glial cell types in just weeks.
- Differentiate your desired iPSC line
- Fast differentiation and maturation
- No genetic footprint
Figure 1. iPSC-derived astrocytes display typical astrocyte morphology and express markers including S100β, CD44, ALDH1L1, and GFAP.
Figure 2. Representative phase contrast images of Quick-Glia™ Astrocyte – SeV Kit cell cultures on days 1, 2, 3, 6, 9, 14, 21, and 28 post-differentiation (scale bar = 100 μm).
Figure 3. Human iPSCs (negative control), human primary astrocytes (hpA, positive control) and Quick-Glia™ Astrocytes at days 42 and 56 post-differentiation were exposed to 100µM L-glutamate for 60 minutes. The amount of glutamate cleared was determined using a Promega Glutamate-Glo assay kit. Bar graph represents 3 biological replicates. Error bars show SD.
Human primary astrocytes (ScienCell Research Laboratories, Catalog number: 1800) and Quick-Glia - Astrocyte SeV kit cultures, harvested at either 28 days or 42 days post SeV infection, were plated at 7,800 cells/cm2 in a Geltrex-coated 96-well plate (Corning, Catalog number: 353072) and grown in ScienCell Astrocyte medium (ScienCell Research Laboratories, without FBS, Catalog number: 1801) for 14 days. As a negative control, human iPSCs were plated in StemFit Basic04 medium (Ajinomoto, Catalog number: SB04) two days before assay measurement using the plating density stated above. 30 minutes prior to assay execution, culture medium was replaced with Hanks' Balanced Salt Solution (HBSS) without phenol red (Fisher Scientific, Catalog number: 14025092). Subsequently, cells were exposed to 100µM L-glutamate (Tocris, Catalog number: 0218) in HBSS for 60 minutes. Immediately after the assay, solution was collected and cells were dislodged using a 1:1 mixture of TrypLE Select Enzyme (Fisher Scientific, Catalog number: 12563029) and 0.02% EDTA (Lonza, Catalog number: 17-711E). The numbers of live cells were determined using a NucleoCounter NC-200 (Chemometec, Catalog number: 900-0200) cell counter. Glutamate concentration was measured by a bioluminescence-based Glutamate-Glo assay kit (Promega, Catalog number: J7021) and a SPECTRAFluor Plus microplate reader (Tecan). To determine the amount of glutamate cleared, all values were first background signal-subtracted using a HBSS only sample and concentrations were thereafter acquired from the glutamate titration curve according to manufacturer’s instructions.
Frequently Asked Questions
Do I need a license for commercial use of Elixirgen’s iPSC glial differentiation kits?
No. The use of glial differentiation kits provided by Elixirgen Scientific does not require any additional license from other parties for any type of use, except for use in humans or for therapeutic or diagnostic use.
What sizes of Quick-Glia™ differentiation kits are available?
Off the shelf, we offer small and large iPSC glial differentiation kits. The small size is best suited for single cell, imaging analyses, and pilot studies while the large size is best for applications such as high throughput screening and RNA sequencing.