iPSC Reagents

iPSC culture media, cell culture substrates, and other reagents we have selected to give the best results when used in conjunction with our Quick-Tissue™ iPSC differentiation kits.

iPSC Media, Cell Culture Substrates, & More

Use the right reagents to get the best results with our differentiation kits. The StemFit® Basic series of iPSC media from Ajinomoto Co. is highly cost-effective and supports the expansion and maintenance of undifferentiated iPSCs. iMatrix-511 and iMatrix-511 silk from Nippi, Inc. are iPSC culture substrates that provide strong adhesion and support long-term self-renewal of iPSCs. We also sell our own coating and dissociation reagents sized to match our differentiation kits for researchers not wanting to invest in full sized reagents for pilot studies.

Advantages

  • Optimized for use with our kits
  • Support growth of undifferentiated PSCs
  • Xeno- and feeder-free iPSC media
Cat No. Product Name Disease StatusDescription
ASB StemFit® Basic Series StemFit® Basic is animal-origin free, defined medium for human iPS/ES cell culture.
NI511 iMatrix-511 series iMatrix-511 series are recommended stem cell culture substrates for maintenance before using Quick-Tissue™ Series.
NCS Neuron Coating Solutions (Small) Solutions for enhancing neuronal cell attachment and adhesion during stem cell differentiation
CDR Cell Dissociation Reagent Reagent for gentle dissociation of undifferentiated and differentiating stem cells.

Frequently Asked Questions

How long do I need to maintain hPSCs prior to beginning to use your kit?

Before beginning differentiation, cultures should be healthy and displaying typical morphologies (i.e., round colonies with tightly packed cells exhibiting a small cytoplasmic area), few spontaneously differentiating cells, and normal growth rates (i.e., doubling once a day). We recommend growing the cells for at least 14 days post-thaw before differentiating and not allowing the cultures to reach more than 80% confluency.

Why do differentiation kits require hPSCs be maintained under StemFit or StemFlex conditions?

hPSCs should be adapted to passaging as single cells prior to differentiation. Our protocols have only been tested with StemFit/StemFlex, so we can't guarantee results using other iPSC media.

Can I use Geltrex or Matrigel as a coating substrate for hPSC cultures?

Our kits are not optimized with these substrates. However, if your hPSC differentiation protocols are already optimized with such a substrate, you may try using one of these with our kit at your own risk.