Gain a deeper understanding of the complexities of cells and tease apart variations due to age, gender, and donor variability with neurons derived from human iPS cells.
Differentiation kit workflow
Representative phase contrast images of Quick-Endothelium™ Vascular – mRNA Kit cell cultures on days 1-5 post-differentiation (scale bar = 100 μm).
Immunofluorescent staining of Quick-Endothelium™ Vascular - mRNA Kit cell cultures shows typical vascular endothelium morphology and expression of CD31 on day 5 post-differentiation (scale bar = 100 μm). Staining conditions: Anti-CD31 primary antibody (Biolegend, Catalog Number: 303102, 1:50 dilution) in combination with a secondary antibody (Invitrogen, Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594, Catalog Number:A11032, 1:500 dilution) were applied. Nuclei were counterstained with Hoechst 33342.
Quick-Endothelium™ Vascular Endothelial cell cultures express both endothelial and tight junction markers and display typical endothelial cell morphology. Immunofluorescent staining that shows expression of endothelial markers CD31 and vWF as well as the tight junction marker Claudin-5 on Day 5. Images courtesy of Ricoh.
Our Quick-Endothelium™ Vascular - mRNA Kits allow researchers to quickly, easily, and efficiently differentiate their iPS or ES cell line of choice into vascular endothelial cells. The kits utilize synthetic messenger RNA (syn-mRNA) to deliver our proprietary cocktail of transcription factors that induce differentiation without leaving a genetic footprint.
| Cat No. | Product Name | Disease StatusDescription |
|---|---|---|
| VE-mRNA | Quick-Endothelium™ Vascular - mRNA Kit | Kit for differentiation of human iPS/ES cells into vascular endothelial cells in 7 days using synthetic mRNA. |
| VE-MM | Quick-Endothelium™ Vascular - Maintenance Medium | Medium for long-term maintenance of human iPS/ESC-derived vascular endothelial cells. |
No. The use of differentiated cells or kits provided by Elixirgen Scientific does not require any additional license from other parties for any type of use, except for use in humans or for therapeutic or diagnostic use. Check out our license statement in Resource section for further detail.
Off the shelf, we offer small (>1 million viable cryopreserved cells) and large (5x >1 million viable cryopreserved cells) sizes. If you are looking for a larger quantity, please contact us for pricing information.
Both delivery methods provide robust “footprint-free,” non-integrating expression of the transcription factors, although each kit uses a slightly different workflow. Based on our data, the differentiation efficiency between an mRNA-based and a SeV-based kit is similar. For example, the percentages of TUBB3+ neurons differentiated using Quick-Neuron™ Cholinergic mRNA- and SeV-based kits were 93% and 88%, respectively.
We recommend the mRNA-based neuron differentiation kits for labs with experience culturing iPSCs/ESCs, as the workflow involves slightly more manipulation of fragile differentiating cells than the SeV-based kits. The SeV-based kits are better for labs with little experience culturing iPSCs/ESCs, although they must be used with BSL-2 safety measures and may require prior approval from your Institutional Biosafety Committee (IBC).
Off the shelf, we offer small and large iPSC neuronal differentiation kits. The small size is best suited for single cell, imaging analyses, and pilot studies while the large size is best for applications such as high throughput screening and RNA sequencing.
