Elixirgen Scientific can help accelerate your research using our proprietary technology to provide fast and reliable differentiation of induced pluripotent stem cells (iPSC) for limitless applications. Human iPSCs revolutionize the study of human biology and your lab can access the power with our services. Using our iPSC services enables researchers to harness the benefits of the latest biomedical research tools.
iPSC Differentiation Service
Consultation Information
Let our brilliant staff create the perfect plan based on your project. You define the scope of your research and we utilize our speedy differentiation technology to provide a customizable iPSC differentiation service to benefit your research. Choose from our wide variety of human patient-derived iPSC lines or we can differentiate your iPSC lines.
Advantages
- ~ Rapid differentiation
- ~ Functionally validated
- ~ No genetic footprint
- ~ Multiple donors and disease types available
How Our Differentiation Service Works
STEP 1
Project Design Consultation
Whether you’re an experienced iPSC researcher or just getting started, our scientists are here to answer your questions and provide ongoing support. All iPSC services begin with a free consultation to define the scope of the project, including the desired differentiated cell type, the production scale (1-50 million viable cells), and desired characterization assays (ICC, MEA, etc.).
If you don’t currently have an iPSC line, we can also provide assistance in obtaining iPSC lines from iPSC banks or gene-editing service providers. Consultations are free, so request a consultation today to discuss your project requirements. iPSC lines Elixirgen recognize are listed here.
STEP 2
iPSC Expansion or Adaptation (Optional)
Upon receiving your frozen or live iPSC line(s), we expand or adapt the cells in optimal conditions for growth and differentiation. For our transcription factor-based differentiation platform, we use feeder-free, single-cell passaging conditions for iPSC expansion/adaptation for best results.
During iPSC expansion, we produce working and back-up stocks of the iPSC line along with a certificate of analysis to verify pluripotency and karyotype upon request.
STEP 3
iPSC Differentiation
Our proprietary iPSC differentiation technology produces functional, highly pure populations neurons in as little as 1 week without leaving a genetic footprint.
Our standard differentiated cell types include a variety of neuronal subtypes (GABAergic, dopaminergic, cholinergic, or sensory) and astrocytes but we can also use our iPSC expertise to develop a custom differentiation strategy for generating your desired cell or tissue type.
STEP 4
Characterization
Prior to shipping, we conduct a variety of quality control checks to ensure that differentiated cells are free from contaminants, express lineage-specific markers, and have optimal post-thaw viability.
We also offer a variety of optional in-house assay services, including microelectrode array (MEA), neurite outgrowth assay, and calcium transient assay, if in depth functional characterization is desired.
Differentiated cells are shipped either live or cryopreserved depending on preference and are supplied with media supplements and detailed instructions for optimal cell maintenance.
Get Started Today!
Choose a better way to study human biology with our services.
Talk with our knowledgeable staff about working together. Whether you know exactly what you need or you want to learn more. Start with our free project design consultation.
Frequently Asked Questions
How much do the iPSC services cost?
The price for the differentiation of one hPSC (iPS or ES) line varies based on multiple factors and requirements such as iPSC expansion requirement, number of cells required, QC criteria etc. Contact us to share your requirements and start discussion.
I don't have my own cells, where can I order an iPSC line?
If you do not already have an established iPSC line, you can find patient iPSC lines in your interest here. Contact us for you to learn more about sourcing iPSC lines and differentiation.
How do I ship my hPSC line?
Sending biomaterials is always challenging. However, Elixirgen Scientific has a lot of experience in cross border logistics for cells and biological materials. Our knowledge and expertise will navigate you to properly prepare your iPSCs to ship. We use dry ice shipment in the US and liquid nitrogen dry shipper internationally to ship cryopreserved cells. We can also accept live hPSC lines to speed up an entire process and lower total fees.
Can you reprogram my cells into iPSCs?
We do not currently offer services for reprogramming cells into iPSCs. However, we can work with our partners to take care of this reprogramming services from fibroblast or PBMC.
